Journal: Polymers

Article Title: Biological Evaluation of Thermosensitive Hydrogels of Chitosan/Hydrolyzed Collagen/β-GP in an In Vitro Model of Induced Cardiac Ischemia

doi: 10.3390/polym16152206

Figure Lengend Snippet: Calcium fluorescence intensities of fetal ventricular cardiomyocytes were recorded at 24 and 72 h post-seeding. ( a ) Healthy cells at 24 h, ( b ) healthy cells at 72 h, ( c ) healthy cells at 24 h intervened with B1 hydrogel, ( d ) healthy cells at 72 h intervened with B1 hydrogel, ( e ) healthy cells at 24 h intervened with B2 hydrogel, ( f ) healthy cells at 72 h intervened with B2 hydrogel. B1: Ch 2.5% w / v /β-GP 10.5% w / v /HcB 6.5% w / v . B2: Ch 2.5% w / v /β-GP 10.5% w / v /HcB 6.5% w / v .

Article Snippet: Finally, the fluorescence intensity was measured for cultures with confluences greater than 75% and with cell densities of 2.5 × 10 4 , which were recorded with an inverted microscope with an OPTIKA brand fluorescence module, model IM-3FL4, and a recording camera brand OPTIKA PRO-5 (Ponteranica, Italy).

Techniques: Fluorescence

Journal: Polymers

Article Title: Biological Evaluation of Thermosensitive Hydrogels of Chitosan/Hydrolyzed Collagen/β-GP in an In Vitro Model of Induced Cardiac Ischemia

doi: 10.3390/polym16152206

Figure Lengend Snippet: Calcium fluorescence intensities of fetal ventricular cardiomyocytes were recorded 24 h post-seeding. ( a ) Ischemic cells (control), ( b ) ischemic cells intervened with B1 hydrogel, ( c ) ischemic cells intervened with encapsulated cells in B1 hydrogel, ( d ) ischemic cells intervened with B2 hydrogel, ( e ) ischemic cells intervened with encapsulated cells in B2 hydrogel. B1: Ch 2.5% w / v /β-GP 10.5% w / v /HcB 6.5% w / v . B2: Ch 2.5% w / v /β-GP 10.5% w / v /HcB 6.5% w / v .

Article Snippet: Finally, the fluorescence intensity was measured for cultures with confluences greater than 75% and with cell densities of 2.5 × 10 4 , which were recorded with an inverted microscope with an OPTIKA brand fluorescence module, model IM-3FL4, and a recording camera brand OPTIKA PRO-5 (Ponteranica, Italy).

Techniques: Fluorescence, Control

Journal: Polymers

Article Title: Biological Evaluation of Thermosensitive Hydrogels of Chitosan/Hydrolyzed Collagen/β-GP in an In Vitro Model of Induced Cardiac Ischemia

doi: 10.3390/polym16152206

Figure Lengend Snippet: Calcium fluorescence intensities of fetal ventricular cardiomyocytes were recorded 72 h post-seeding. ( a ) Ischemia control, ( b ) Ischemic cells intervened with B1 hydrogel, ( c ) Ischemic cells intervened with encapsulated cells in B1 hydrogel, ( d ) Ischemic cells intervened with the B2 hydrogel, ( e ) Ischemic cells intervened with encapsulated cells in B2 hydrogel. B1: Ch 2.5% w / v /β-GP 10.5% w / v /HcB 6.5% w / v . B2: Ch 2.5% w / v /β-GP 10.5% w / v /HcB 6.5% w / v .

Article Snippet: Finally, the fluorescence intensity was measured for cultures with confluences greater than 75% and with cell densities of 2.5 × 10 4 , which were recorded with an inverted microscope with an OPTIKA brand fluorescence module, model IM-3FL4, and a recording camera brand OPTIKA PRO-5 (Ponteranica, Italy).

Techniques: Fluorescence, Control